Broad specificity profiling of TALENs results in engineered nucleases with improved DNA-cleavage specificity
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چکیده
منابع مشابه
Rationally engineered Cas9 nucleases with improved specificity.
The RNA-guided endonuclease Cas9 is a versatile genome-editing tool with a broad range of applications from therapeutics to functional annotation of genes. Cas9 creates double-strand breaks (DSBs) at targeted genomic loci complementary to a short RNA guide. However, Cas9 can cleave off-target sites that are not fully complementary to the guide, which poses a major challenge for genome editing. ...
متن کاملRationally engineered Cas 9 nucleases with improved specificity
1. A. M. Zaki, S. van Boheemen, T. M. Bestebroer, A. D. Osterhaus, R. A. Fouchier, N. Engl. J. Med. 367, 1814–1820 (2012). 2. A. Bermingham et al., Euro Surveill. 17, 20290 (2012). 3. World Health Organization, Middle East Respiratory Syndrome Coronavirus (MERS-CoV) (2015); www.who.int/emergencies/ mers-cov/en/. 4. C. B. E. M. Reusken et al., Lancet Infect. Dis. 13, 859–866 (2013). 5. A. N. Ala...
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tRNAs in eukaryotic nuclei and organelles are synthesized as precursors lacking the 3'-terminal CCA sequence and possessing 5' (leader) and 3' (trailer) extensions. Nucleolytic cleavage of the 3' trailer and addition of CCA are therefore required for formation of functional tRNA 3' termini. Many chloroplast tRNA genes encode a C at position 74 which is not removed during processing but which ca...
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MicroRNAs (miRNAs) have quickly emerged as important regulators of mammalian physiology owing to their precise control over the expression of critical protein coding genes. Despite significant progress in our understanding of how miRNAs function in mice, there remains a fundamental need to be able to target and edit miRNA genes in the human genome. Here, we report a novel approach to disrupting...
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Sir: The bleomycins (BLM’s) are a family of structurally complex glycoprotein antibiotics possessing significant anticancer activity ( I ) . They have been demonstrated to effect DNA strand scission in a reaction that is both metal ion and oxygen dependent (2); this transformation is believed to form the basis for the therapeutic efficacy of the bleomycins. A number of studies have demonstrated...
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ژورنال
عنوان ژورنال: Nature Methods
سال: 2014
ISSN: 1548-7091,1548-7105
DOI: 10.1038/nmeth.2845